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hfob cells procell  (Procell Inc)

 
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    Procell Inc hfob cells procell
    Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells <t>(hFOB,</t> U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B <t>cells</t> <t>cultured</t> on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm
    Hfob Cells Procell, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hfob cells procell/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    hfob cells procell - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma"

    Article Title: Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

    Journal: Bone Research

    doi: 10.1038/s41413-023-00265-w

    Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm
    Figure Legend Snippet: Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm

    Techniques Used: Transformation Assay, Expressing, Quantitative Proteomics, Western Blot, Staining, Cell Culture, Derivative Assay



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    Procell Inc hfob cells procell
    Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells <t>(hFOB,</t> U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B <t>cells</t> <t>cultured</t> on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm
    Hfob Cells Procell, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hfob cells procell/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    hfob cells procell - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm

    Journal: Bone Research

    Article Title: Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

    doi: 10.1038/s41413-023-00265-w

    Figure Lengend Snippet: Toggling between normal and transformed growth based on sensor proteins. a Reductions in sensor protein expression levels in transformed cells (HOS and 143B) compared to nontransformed cells (hFOB, U2OS, and MG63), as determined by quantitative proteomics. n = 3 in each group; the data are presented as the means. Representative Western blots of sensor proteins (TPM1, TPM2, and TPM3) in U2OS and 143B cells ( b ) and quantification ( c ). n = 3 in each group; the data are presented as the mean ± s.d. values. Staining for paxillin (green) in TPM1 - and TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on glass surfaces overnight ( d ) and quantification ( e , f ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Staining for actin (red) in TPM1 - or TPM2 -silenced U2OS cells and TPM3 -silenced 143B cells cultured on rigid (40 kPa) and soft (4 kPa) hydrogel surfaces overnight ( g ) and quantification ( h , i ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Soft agar cultures of TPM3 -silenced 143B cells for 2 weeks ( j ) and quantification ( k , l ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. Sphere cultures of TPM3 -silenced 143B cells propagated for 7 days ( m ) and quantification ( n ). n = 5 fields in each group; the data are presented as the mean ± s.d. values. The graphs show the individual data points derived from three independent measurements and the means. * P < 0.05; ** P < 0.01; **** P < 0.000 1. Scale bars, 50 μm

    Article Snippet: hFOB cells (Procell) were cultured at 34 °C in a 5% CO 2 incubator in Dulbecco’s modified Eagle’s medium/F12 supplemented with 10% fetal bovine serum (FBS).

    Techniques: Transformation Assay, Expressing, Quantitative Proteomics, Western Blot, Staining, Cell Culture, Derivative Assay